Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
- Order Number
- Package Size
- 100 µl
The prognosis and clinical course of CLL are heterogeneous. Conventional banding techniques in CLL are hampered by the low mitotic index of the neoplastic cells. The introduction of interphase cytogenetics using fluorescent in situ hybridization (FISH) has greatly increased the sensitivity of cytogenetic analyses. With FISH abnormalities can be detected in more than 80 % of patients by using a 4-probe panel for the detection of trisomy 12q13-15, and deletions 13q14, 17p13, and 11q22-23. An additional 10 % of patients can be shown to carry a 6q21 deletion, 14q32 translocation, and partial trisomy 3q or 8q.
The most frequently deleted region in B-CLL is located in 13q14.3 distal to RB1. The minimal deleted region (MDR) is 350 kb in size and includes the gene loci for the mi-RNAs 15 and 16. CLL with 13q deletion as the sole cytogenetic abnormality usually have a good prognosis.
Deletions of chromosomal region 13q14 have also been reported in a variety of human tumors, including other types of lymphoid tumors and myeloid leukemias, as well as prostate, head and neck, and non-small-cell lung cancers.
- Chronic Lymphocytic Leukemia (CLL)
- Multiple Myeloma and Plasma Cell Neoplasms (MM)
Normal Cell: Two green (2G), two orange (2O), and two blue (2B) signals.
Three green (3G), two orange (2O), and two blue (2B) signals.
Aberrant Cell: Two green (2G), one orange (1O), and two blue (2B) signals.
Aberrant Cell: Two green (2G), one orange (1O), and one blue (1B) signal.
- Doehner et al (2000) N Engl J Med 34:1910-1916
- DeWald et al (2003) Brit J Haem 121:287-295
- Nelson at al (2007) Am J Clin Pathol 128:323-332