XL DLEU/LAMP/12cen

Deletion/Enumeration Probe

Order Number
D-5055-100-TC
Package Size
100 µl
Labels
   
Chromosomes
1312

Description

XL DLEU/LAMP/12cen

The XL DLEU/LAMP/12cen is a 3-color probe that hybridizes to the DLEU gene region at 13q14 (including the D13S319 marker) in orange, the LAMP gene region at 13q34 in blue (aqua), and a chromosome 12 centromeric probe labeled in green.

Clinical Details

The prognosis and clinical course of CLL are heterogeneous. Conventional banding techniques in CLL are hampered by the low mitotic index of the neoplastic cells. The introduction of interphase cytogenetics using fluorescent in situ hybridization (FISH) has greatly increased the sensitivity of cytogenetic analyses. With FISH abnormalities can be detected in more than 80 % of patients by using a 4-probe panel for the detection of trisomy 12q13-15, and deletions 13q14, 17p13, and 11q22-23. An additional 10 % of patients can be shown to carry a 6q21 deletion, 14q32 translocation, and partial trisomy 3q or 8q.

The most frequently deleted region in B-CLL is located in 13q14.3 distal to RB1. The minimal deleted region (MDR) is 350 kb in size and includes the gene loci for the mi-RNAs 15 and 16. CLL with 13q deletion as the sole cytogenetic abnormality usually have a good prognosis.

Deletions of chromosomal region 13q14 have also been reported in a variety of human tumors, including other types of lymphoid tumors and myeloid leukemias, as well as prostate, head and neck, and non-small-cell lung cancers.

Clinical Applications

  • Chronic Lymphocytic Leukemia (CLL)
  • Multiple Myeloma and Plasma Cell Neoplasms (MM)

Images

XL DLEU/LAMP/12cen

XL DLEU/LAMP/12cen hybridized to bone marrow cells. Three interphases are shown of which one shows a normal signal pattern of 2 orange, 2 green, and 2 blue signals, one nucleus shows a hemizygous, and a third one a homozygous deletion of DLEU (orange).

Expected Patterns

Expected Pattern 1

Normal Cell: Two green (2G), two orange (2O), and two blue (2B) signals.

Expected Pattern 2

Aberrant Cell:
Three green (3G), two orange (2O), and two blue (2B) signals.

Expected Pattern 3

Aberrant Cell: Two green (2G), one orange (1O), and two blue (2B) signals.

Expected Pattern 4

Aberrant Cell: Two green (2G), one orange (1O), and one blue (1B) signal.

Literature

  • Doehner et al (2000) N Engl J Med 34:1910-1916
  • DeWald et al (2003) Brit J Haem 121:287-295
  • Nelson at al (2007) Am J Clin Pathol 128:323-332

Downloads

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