Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
XL IGH plus
Break Apart Probe
- Order Number
- Package Size
- 100 µl
Chromosomal translocations affecting the IGH locus are recurrent in many types of lymphomas. The malignant transformation works via the juxtaposition of oncogenes next to regulatory sequences of the immunoglobulin locus.
Due to the telomeric position of the IgH locus, 14q32.3 translocations may be easily missed by conventional cytogenetics. A break apart IGH probe covering the central IGHV region and located proximally from IGHJ close to the JAG2 gene, allows to identify most IGH rearrangements independent of breakpoint variations.
- Multiple Myeloma and Plasma Cell Neoplasms (MM)
- Non-Hodgkin Lymphomas (NHL)
- Acute Lymphoblastic Leukemia (ALL)
Two green-orange (2GO) fusion signals representing the two normal IGH loci.
Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signal, indicating a chromosome break in the IGH locus.
- Martin-Subero et al (2006) Cancer Res 66:10332-10338
- Wlodarska et al (2007) J Mol Diagn 9:47-54
- Dyer et al (2010) Blood 115:1490-1499