XL IGH plus

Break Apart Probe

Order Number
D-5061-100-OG
Package Size
100 µl
Labels
  
Chromosome
14

Description

XL IGH plus

This product has been replaced by XL IGH BA. Order Number D-5107-100-OG

Clinical Details

Chromosomal translocations affecting the IGH locus are recurrent in many types of lymphomas. The malignant transformation works via the juxtaposition of oncogenes next to regulatory sequences of the immunoglobulin locus.

Due to the telomeric position of the IgH locus, 14q32.3 translocations may be easily missed by conventional cytogenetics. A break apart IGH probe covering the central IGHV region and located proximally from IGHJ close to the JAG2 gene, allows to identify most IGH rearrangements independent of breakpoint variations.

Clinical Applications

  • Multiple Myeloma and Plasma Cell Neoplasms (MM)
  • Non-Hodgkin Lymphomas (NHL)
  • Acute Lymphoblastic Leukemia (ALL)

Images

XL IGH plus

XL IGH plus hybridized to lymphocytes. One normal interphase and metaphase are shown.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green-orange (2GO) fusion signals representing the two normal IGH loci.

Expected Pattern 2

Aberrant Cell (typical results):
One green (1G), one orange (1O), and one green-orange (1GO) fusion signal, indicating a chromosome break in the IGH locus.

Literature

  • Martin-Subero et al (2006) Cancer Res 66:10332-10338
  • Wlodarska et al (2007) J Mol Diagn 9:47-54
  • Dyer et al (2010) Blood 115:1490-1499

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