Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
Translocation/Dual Fusion Probe
- Order Number
- Package Size
- 100 µl
Genetic aberrations present in multiple myeloma cells play a significant role in the risk stratification and therapeutic approach in multiple myeloma patients. Chromosomal translocations affecting the IGH locus are recurrent in many types of leukemias and lymphomas.The malignant transformation works via the juxtaposition of oncogenes next to regulatory sequences of the immunoglobulin locus.
The t(11;14)(q13;q32) results in up-regulation of cyclin D1 and is the most common translocation detected in multiple myeloma. This translocation represents a unique subset of patients with a relatively favorable outcome. The t(11;14) is also the genetic hallmark of mantle cell lymphoma.
- Multiple Myeloma and Plasma Cell Neoplasms (MM)
- Non-Hodgkin Lymphomas (NHL)
- Chronic Lymphocytic Leukemia (CLL)
Two green (2G) and two orange signals (2O).
Aberrant Cell (typical results):
One green (1G), one orange (1O), and two green-orange fusion signals (2GO).
- Jansen et al (2000) Blood 95:2691-2698
- Fonseca et al (2002) Blood 99:3735-3741
- Sander et al (2008) Haematologica 93:680-687