Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
Translocation/Dual Fusion Probe
- Order Number
- Package Size
- 100 µl
Genetic aberrations present in multiple myeloma cells play a significant role in the risk stratification and therapeutic approach in multiple myeloma patients. Chromosomal translocations affecting the IGH locus are recurrent in many types of leukemias and lymphomas.The malignant transformation works via the juxtaposition of oncogenes next to regulatory sequences of the immunoglobulin locus.
The t(4;14)(p16;q32) is a primary event in multiple myeloma (MM) and deregulates the expression of the fibroblast growth factor receptor 3 (FGFR3) and MMSET. Multivariate analysis has identified t(4;14) as the only adverse prognostic factor for both progression-free survival and overall survival.
- Multiple Myeloma and Plasma Cell Neoplasms (MM)
Two green (2G) and two orange signals (2O).
Aberrant Cell (typical results):
One green (1G), one orange (1O), and two green-orange fusion signals (2GO).
- Chesi et al (1998) Blood 92:3025-3034
- Chang et al (2004) Br J Haematol 125:64-68
- Saez et al (2007) Onco Reports 18:1099-1106