Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
XL t(14;18) IGH/BCL2
Translocation/Dual Fusion Probe
- Order Number
- Package Size
- 100 µl
Follicular lymphomas (FL) and diffuse large cell lymphoma (DLBCL) represent the most common entities of non-Hodgkin's lymphoma (NHL) worldwide with up to 29 % and 37 % of B-cell lymphomas.
The translocation t(14;18) juxtaposes the IGH enhancer region with the BCL-2 oncogen at 18q21 and is present in 70 % to 95 % of follicular lymphomas. By this rearrangement BCL-2 gene expression is deregulated, leading to overproduction of the antiapoptotic protein BCL-2. The same aberration is present in 20 - 30 % of diffuse large cell lymphomas.
- Non-Hodgkin Lymphomas (NHL)
Two green (2G) and two orange signals (2O).
Aberrant Cell (typical results):
One green (1G), one orange (1O), and two green-orange fusion signals (2GO).
- Einerson et al (2005) Am J Clin Pathol 124:421-429
- Weinberg et al (2007) J Mol Diagn 9:530-537
- Gu et al (2008) Arch Pathol Lab Med 132:1355-1361