XL t(15;17) DF

Translocation/Dual Fusion Probe

Order Number
D-5086-100-OG
Package Size
100 µl
Labels
  
Chromosomes
1517

Description

XL t(15;17) DF

The XL t(15;17) DF is designed as a dual fusion probe. An orange labeled probe flanks the breakpoint at the PML gene at 15q24. A green labeled probe spans RARA and flanking regions.

Clinical Details

Several recurrent balanced translocations and inversions, and their variants, are recognized in the WHO category acute myeloid leukemia (AML) with recurrent genetic abnormalities. Furthermore, several cytogenetic abnormalities are considered sufficient to establish the WHO diagnosis of AML with myelodysplasia-related features when 20% or more blood or marrow blasts are present.

AML M3 and AML M3v are characterized by a reciprocal translocation between the long arm of chromosome 15 and the long arm of chromosome 17. This translocation leads to a rearrangement of the PML gene situated on chromosomal band 15q24 and the RARA gene situated on band 17q21. The PML-RARA rearrangement has gained major clinical importance because in combination with all-trans retinoic acid (ATRA) and conventional anthracycline and cytarabine based chemotherapy it leads to an improved prognosis in this subgroup of AML.

Clinical Applications

  • Acute Myelogenous Leukemia (AML)

Images

XL t(15;17) DF

XL t(15;17) DF hybridized to bone marrow cells. One orange, one green, and two fusion signals each are observed in the interphase nuclei indicating the presence of t(15;17).

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green (2G) and two orange (2O) signals.

Expected Pattern 2

Aberrant Cell (typical results):
One green (1G), one orange (1O), and two green-orange colocalization/fusion signals (2GO) resulting from a reciprocal translocation between the relevant loci.

Literature

  • Grimwade et al (2000) Blood 96:1297-1308
  • Schoch et al (2002) Hematol J 3:259-263
  • Campbell et al (2013) BioMed Res Int: Article ID 164501

Downloads

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