Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
XL t(6;14) CCND3/IGH DF
Translocation/Dual Fusion Probe
- Order Number
- Package Size
- 100 µl
The most frequent primary abnormalities in multiple myeloma (MM) are trisomies of odd-numbered chromosomes or translocations involving the immunglobulin heavy chain (IGH) gene locus. The most common MM-associated IGH translocations are t(11;14), t(4;14), t(6;14), t(14;16) and t(14;20) in the order of their occurrence. The consequence of these rearrangements is the dysregulation of genes juxtaposed to transcriptional enhancers in the IGH locus. Prognosis and risk stratification strongly depends on the detection and interpretation of cytogenetic primary abnormalities. t(14;16) and t(14;20) are considered as high risk, t(4;14) as intermediate risk and t(6;14) and t(11;14) as standard risk cytogenetic aberrations in patients with MM based on FISH testing. Secondary aberrations are also influencing the outcome.
Cyclins of the D-family are essential for the transition of the G1 to the S-Phase during the cell cycle. t(6;14)(p21;q32) moves the cyclin D3 gene in proximity to 3´ IGH enhancer sequences and is associated with cyclin D3 overexpression. The chromosomal translocation has been reported as a rare and recurrent event not only in myeloma but also in other B-cell malignancies as diffuse large B-cell lymphoma.
- Multiple Myeloma and Plasma Cell Neoplasms (MM)
Two green (2G) and two orange (2O) signals.
Aberrant Cell (typical results):
One green (1G), one orange (1O), and two green-orange colocalization/fusion signals (2GO) resulting from a reciprocal translocation between the relevant loci.
- Shaughnessy et al (2001) Blood 98:217-223
- Sonoki et al (2001) Blood 98:2837-2844
- Rajan and Rajkumar (2015) Blood Cancer J. 5:e365