XL t(8;21) plus

Translocation/Dual Fusion Probe

Order Number
D-5114-100-OG
Package Size
100 µl
Labels
  
Chromosomes
821

Description

XL t(8;21) plus

The XL t(8;21) plus is designed as a dual fusion probe. The orange labeled probe spans the breakpoint at 21q22.1 RUNX1 (formerly AML1), and the green labeled probe spans the breakpoint at 8q21 RUNX1T1 (formerly ETO).

Clinical Details

Several recurrent balanced translocations and inversions, and their variants, are recognized in the WHO category acute myeloid leukemia (AML) with recurrent genetic abnormalities. Furthermore, several cytogenetic abnormalities are considered sufficient to establish the WHO diagnosis of AML with myelodysplasia-related features when 20% or more blood or marrow blasts are present.

The t(8;21)(q21;q22) is the most common translocation in de novo AML occurring in up to 20% of adult and 40% of pediatric cases of AML. The translocation fuses RUNX1 with RUNX1T1 to produce a RUNX1/RUNX1T1 fusion gene located on the derivative chromosome 8. The prognosis after intensive chemotherapy is better for these patients than for the majoritiy of AML patients. Small hidden interstitial insertions resulting in an RUNX1/RUNX1T1 rearrangement have been found, necessitating the use of a breakpoint spanning rather than a breakpoint flanking FISH probe.

Clinical Applications

  • Acute Myelogenous Leukemia (AML)

Images

XL t(8;21) plus

XL t(8;21) plus hybridized to lymphocytes. One normal metaphase and one normal interphase are shown.

Expected Patterns

Expected Pattern 1

Normal Cell:
Two green (2G) and two orange (2O) signals.

Expected Pattern 2

Aberrant Cell (typical results):
One green (1G), one orange (1O), and two green-orange colocalization/fusion signals (2GO) resulting from a reciprocal translocation between the relevant loci.

Literature

  • Zhang et al (2002) PNAS 99: 3070-3075
  • Gamerdinger et al (2003) Gene Chromosome Canc 36: 261-272
  • Jang et al (2010) Ann Clin Lab Sci 40 :80-84

Downloads

Order Now or Request Assistance

Contact Us »

Contact MetaSystems directly or find your local MetaSystems partner.