About 100 guests from 36 countries met on the XVIII. MetaSystems Distributor Meeting (DM) in November to exchange experiences and to get to know new trends and developments at MetaSystems.
XL t(4;11) AFF1/KMT2A DF
Translocation/Dual Fusion Probe
- Order Number
- Package Size
- 100 µl (10 Tests)
XL t(4;11) AFF1/KMT2A DF consists of an orange-labeled probe hybridizing to the AFF1 gene region at 4q21.3-22 and a green-labeled probe hybridizing to the KMT2A gene region at 11q23.3.
Probe maps are created in accordance with the intended purpose of the product. Solid colored bars do not necessarily indicate that the probe fully covers the indicated genomic region. Therefore, caution is advised when interpreting results generated through off-label use. Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale. Further information is available on request.
Chromosomal rearrangements of the KMT2A (lysine methyltransferase 2A) gene, formerly MLL (mixed lineage leukemia), are associated with various hematological disorders. Most patients suffer from acute myeloid leukemia (AML) or acute lymphoblastic leukemia (ALL), while only a minority develops mixed lineage leukemia (MLL). Several chromosomal aberrations involving the KMT2A gene have been identified. However, the majority of leukemias result from translocations leading to KMT2A fusions. More than 90 KMT2A translocation partner genes fused to the 5´- KMT2A portion have been identified. The most common translocation partners in KMT2A associated leukemia are AFF1, MLLT3, MLLT1, MLLT10, ELL and AFDN, described here in the order of their frequency. The fusion of KMT2A and AFF1 (AF4/FMR2 family member 1) is the most frequent KMT2A translocation observed in ALL cases. About 60% of ALL patients carrying KMT2A rearrangements are characterized by the KMT2A-AFF1 fusion gene. Infant patients mainly have breakpoints in intron 11 of KMT2A, whereas adult patients have a tendency for breakpoints within intron 9. Oncogenic KMT2A-AFF1 fusions have been implicated in the DOT1L and SEC transcriptional complexes. AFF1 plays an important role in the regulation of RNA polymerase II-dependent transcription, elongation and chromatin remodeling mediated by histone methylation. AFF1 is the docking platform for several components of the DOT1L complex consisting of DOT1L, a H3K79 methyltransferase, MLLT3, MLLT1, and MLLT10, whose genes are also common translocation partners of KMT2A. Mouse studies revealed increased and extended histone methylation signatures in the presence of KMT2A-AFF1 and reciprocal AFF1-KMT2A fusions. DOT1L inhibitors are promising candidates for clinical treatment which are currently being evaluated.
- Acute Lymphoblastic Leukemia (ALL)
Two green (2G) and two orange (2O) signals.
Aberrant Cell (typical results):
One green (1G), one orange (1O), and two green-orange colocalization/fusion signals (2GO) resulting from a reciprocal translocation between the respective loci.
- De Braekeleer et al (2011) Mol Oncol 5:555-563
- Meyer et al (2013) Leukemia 27:2165-2176
- Burns et al (2018) Hematology (7th ed) Ch 64: Pathobiology of acute lymphoblastic leukemia:1005–1019.e11