XL t(4;11) AFF1/KMT2A DF

Translocation/Dual Fusion Probe

Order Number
D-5131-100-OG
Package Size
100 µl (10 Tests)
Labels
  
Chromosomes
411
Regulatory Status
IVDD

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Product Description

XL t(4;11) AFF1/KMT2A DF

XL t(4;11) AFF1/KMT2A DF consists of an orange-labeled probe hybridizing to the AFF1 gene region at 4q21.3-22 and a green-labeled probe hybridizing to the KMT2A gene region at 11q23.3.

Probe maps are created in accordance with the intended purpose of the product. Solid colored bars do not necessarily indicate that the probe fully covers the indicated genomic region. Therefore, caution is advised when interpreting results generated through off-label use. Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale. Further information is available on request.

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Clinical Details

Chromosomal rearrangements of the KMT2A (lysine methyltransferase 2A) gene, formerly MLL (mixed lineage leukemia), are associated with various hematological disorders. Most patients suffer from acute myeloid leukemia (AML) or acute lymphoblastic leukemia (ALL), while only a minority develops mixed lineage leukemia (MLL). Several chromosomal aberrations involving the KMT2A gene have been identified. However, the majority of leukemias result from translocations leading to KMT2A fusions. More than 90 KMT2A translocation partner genes fused to the 5´- KMT2A portion have been identified. The most common translocation partners in KMT2A associated leukemia are AFF1, MLLT3, MLLT1, MLLT10, ELL and AFDN, described here in the order of their frequency. The fusion of KMT2A and AFF1 (AF4/FMR2 family member 1) is the most frequent KMT2A translocation observed in ALL cases. About 60% of ALL patients carrying KMT2A rearrangements are characterized by the KMT2A-AFF1 fusion gene. Infant patients mainly have breakpoints in intron 11 of KMT2A, whereas adult patients have a tendency for breakpoints within intron 9. Oncogenic KMT2A-AFF1 fusions have been implicated in the DOT1L and SEC transcriptional complexes. AFF1 plays an important role in the regulation of RNA polymerase II-dependent transcription, elongation and chromatin remodeling mediated by histone methylation. AFF1 is the docking platform for several components of the DOT1L complex consisting of DOT1L, a H3K79 methyltransferase, MLLT3, MLLT1, and MLLT10, whose genes are also common translocation partners of KMT2A. Mouse studies revealed increased and extended histone methylation signatures in the presence of KMT2A-AFF1 and reciprocal AFF1-KMT2A fusions. DOT1L inhibitors are promising candidates for clinical treatment which are currently being evaluated.

Clinical Applications

  • Acute Lymphoblastic Leukemia (ALL)
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Images

XL t(4;11) AFF1/KMT2A DF

XL t(4;11) AFF1/KMT2A DF hybridized to lymphocytes. Three normal interphases are shown.

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Expected Patterns

Expected Pattern 1

Normal Cell:
Two green (2G) and two orange (2O) signals.

Expected Pattern 2

Aberrant Cell (typical results):
One green (1G), one orange (1O), and two green-orange colocalization/fusion signals (2GO) resulting from a reciprocal translocation between the respective loci.

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Literature

  • De Braekeleer et al (2011) Mol Oncol 5:555-563
  • Meyer et al (2013) Leukemia 27:2165-2176
  • Burns et al (2018) Hematology (7th ed) Ch 64: Pathobiology of acute lymphoblastic leukemia:1005–1019.e11

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News

IVDR Certification

MetaSystems Probes has received IVDR certification for our initial 26 fluorescence in situ hybridization (FISH) probes from the notified body, BSI. Achieving this milestone was not without its challenges, and we are delighted to have accomplished IVDR certification for this probe set at an early stage.

IVDR Certification

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MetaSystems Probes is pleased to announce the new catalog including new and innovative products and supporting information. A PDF version of the new catalog is available for download here.

New Probes Catalog is Online!