Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
XL 21q22 / XCP 21
- Order Number
- Package Size
- 50 µl
This probe is not intended for use to analyze trisomy 21 in prenatal diagnosis.
XL 21q22/XCP 21 hybridized to normal human metaphase spreads will result in green signals along the entire length of chromosome 21 combined with orange signals at 21q22 (Down Syndrome Critical Region 4). A translocation with involvement of chromosome 21 will result in a split of a green signal. In this case the green signal will be partially visible on the der(21) and the respective partner chromosome of the translocation. Depending on the localization of the breakpoint on the der(21), the signal of the locus-specific probe remains on the der(21) or will be observed on the translocation partner.