Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
Translocation/Dual Fusion Probe
- Order Number
- Package Size
- 100 µl
Several recurrent balanced translocations and inversions, and their variants, are
recognized in the WHO category acute myeloid leukemia (AML) with recurrent genetic abnormalities. Furthermore, several cytogenetic abnormalities are considered sufficient to establish the WHO diagnosis of AML with myelodysplasia-related features when 20% or more blood or marrow blasts are present.
The t(8;21)(q22;q22) is the most common translocation in de novo AML occurring in up to 20 % of adult and 40 % of pediatric cases of AML. The translocation fuses AML1 (RUNX1) with ETO (RUNX1T1) to produce the AML1/ETO fusion gene located on the derivative 8 chromosome. The prognosis after intensive chemotherapy is better for these patients than for the majority of AML patients.
- Acute Myelogenous Leukemia (AML)
Normal Cell: Two green (2G) and two orange (2O) signals.
Aberrant Cell (typical results):
One green (1G), one orange (1O), and two green-orange signals (2GO).
- Levanon et al (2001) Gene 262:23-33
- Varella-Garcia et al (2001) Leukemia 15:1408-1414
- ? Zhang et al (2002) PNAS 99: 3070-3075