Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
Translocation/Dual Fusion Probe
- Order Number
- Package Size
- 100 µl
Chromosomal translocations affecting the IGH locus are recurrent in many types of lymphomas. The malignant transformation works via the juxtaposition of oncogenes next to regulatory sequences of the immunoglobulin locus.
Aberrant expression of MAFB is reported to be involved in the oncogenic transformation of myeloma cells that harbor the t(14;20). It can be found in about 2 % of multiple myeloma and less frequent in monoclonal gammopathy of undetermined significance (MGUS) patients. Cases characterized by this translocation can be stable for years before progression occurs.
- Multiple Myeloma and Plasma Cell Neoplasms (MM)
Two green (2G) and two orange signals (2O).
Aberrant Cell (typical results):
One green (1G), one orange (1O), and two green-orange fusion signals (2GO).
- Boersma-Vreugdenhil et al (2004) Brit J Haem 126:355-363
- Zhan et al (2006) Blood 108:2020-2028
- Ross et al (2010) Haematologica 95:1221-1225