XL t(12;21) ETV6/RUNX1 DF

Translocation/Dual Fusion Probe

Order Number
D-5115-100-OG
Package Size
100 µl
Labels
  
Chromosomes
1221

Description

XL t(12;21) ETV6/RUNX1 DF

XL t(12;21) ETV6/RUNX1 DF consists of a green-labeled probe hybridizing to the ETV6 gene region at 12p13.2 and an orange-labeled probe hybridizing to the RUNX1 gene region at 21q22.1.

Probe maps are created in accordance with the intended purpose of the product. Solid colored bars do not necessarily indicate that the probe fully covers the indicated genomic region. Therefore, caution is advised when interpreting results generated through off-label use. Probe map details based on UCSC Genome Browser GRCh37/hg19. Map components not to scale. Further information is available on request.

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Clinical Details

Acute lymphoblastic leukemia (ALL) is a rapidly progressing cancer type characterized by the malignant transformation of lymphoid progenitor cells. It is the most common childhood cancer type and the second most common leukemia in adults. Treatment of children usually results in good prognosis whereas the outcome for adults is less optimistic. Most patients show a transformation of precursors of the B-cell type, but also the T-cell phenotype is frequently observed. The most common aberration in pediatric B-cell ALL is t(12;21)(p13;q22) with an incidence of about 25%, compared to <5% in adults. The result of this reciprocal translocation is an ETV6/RUNX1 fusion gene. Scientific data suggest that ETV6/RUNX1 is already established prenatally, but additional chromosomal aberrations are necessary for the development of ALL postnatally. The ETV6/RUNX1 fusion gene is transcriptionally active and is dysregulating a cascade of downstream genes. One study has shown, that all positive t(12;21) cases harbored the ETV6/RUNX1 fusion gene but not the reciprocal gene. This suggests, that ETV6/RUNX1 is involved in the manifestation of ALL, but not RUNX1/ETV6.

Since t(12;21) is not detectable by conventional cytogenetic methods, FISH is one of the methods of choice.

Clinical Applications

  • Acute Lymphoblastic Leukemia (ALL)
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Images

XL t(12;21) ETV6/RUNX1 DF

XL t(12;21) ETV6/RUNX1 DF hybridized to bone marrow cells. One normal interphase and one aberrant interphases are shown. The reciprocal translocation is splitting the orange and green signals, resulting in two fusion signals on the relevant chromosomes. One green loci is deleted. The relevant normal remaining loci is indicated by one orange signal.

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Expected Patterns

Expected Pattern 1

Normal Cell:
Two green (2G) and two orange (2O) signals.

Expected Pattern 2

Aberrant Cell (typical results):
One green (1G), one orange (1O), and two green-orange colocalization/fusion signals (2GO) resulting from a reciprocal translocation between the relevant loci.

Expected Pattern 3

Aberrant Cell (typical results):
No green (no G), one orange (1O), and two green-orange colocalization/fusion signals (2GO) resulting from a reciprocal translocation between the respective loci and a deletion of the locus covered by the green probe. In t(12;21) cases the normal ETV6 at 12p13 is often deleted.

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Literature

  • Romana et al (1995) Blood 85:3662-3670
  • Al-Obaidi et al (2002) Leukemia 16:669-674
  • Sun et al (2017) Oncotarget 8:35445-35459

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