Fluorescence in situ hybridization has become an essential detection assay in today´s routine diagnostics. However, long hybridization times of many hours to overnight are still a restrictive factor. We have refined the production process of our FISH probes to reduce background and artefacts and to improve the signal to noise ratio, particularly in short-time hybridization. Since mid-2015, one hour hybridization on lymphocytes is an integral part of quality control for all XCyting locus-specific probes at our manufacturing facility.
Translocation/Dual Fusion Probe
- Order Number
- Package Size
- 100 µl
A number of recurrent chromosomal abnormalities have been shown to have prognostic significance in acute lymphoblastic leukemia, especially in B-precursor ALL. Some chromosomal abnormalities, such as high hyperdiploidy and the ETV6-RUNX1 fusion, are associated with more favorable outcomes, while others, including the t(9;22), rearrangements of the KMT2A gene (chromosome 11q23), and intrachromosomal amplification of the AML1 gene (iAMP21), are associated with a worse prognosis.
The most common translocation is the t(12;21)(p13;q22), which is recognized in up to 25 % of B-precursor ALL. This translocation fuses ETV6 with the RUNX1 gene. The resulting fusion transcript is a transcription factor and functions as a corepressor at RUNX1 target genes. The ETV6/RUNX1 translocation generally implies a good prognosis.
- Acute Lymphoblastic Leukemia (ALL)
Normal Cell: Two green (2G) and two orange signals (2O).
Aberrant Cell (typical results):
One green (1G), one orange (1O), and two green-orange fusion signals (2GO).
Aberrant Cell (typical results): One orange (1O), and two green-orange fusion (2GO) (adjacent green and orange) signals. In t(12;21) cases the normal ETV6 at 12p13 is often deleted.
- Romana et al (1995) Blood 85:3662-3670
- Sato et al (1997) Blood 90:4886-4893